Featured Publications Authored by Vireo Advisors, LLC

Alternative Proteins, Cultured Meat and Seafood, Novel, Healthy, and Sustainable Foods

• Food Safety of Fermented Proteins and Cultivated Meat and Seafood, Chapter 6 in Cellular Agriculture Technology, Society, Sustainability and Science, Ong, K.J., Case, F.H., and Shatkin, J.A. (2023). Published by Elsevier.

• Cultured Meat Safety Research Priorities: Regulatory and Governmental Perspectives, Ong, K.J., Tejeda-Saldana, Y., Duffy, B., Holmes, D., Kukk, K., Shatkin, J.A. (2023). Foods 12, no. 14: 2645.

• Food Safety Considerations and Research Priorities for the Cultured Meat and Seafood Industry, Ong, K.J., Johnston, J., Datar, I., Sewalt, V., Holmes, D. and Shatkin, J.A. (2021). . Comprehensive Reviews Food Sci. Food Safety.

• Physical, chemical, and toxicological characterization of sulfated cellulose nanocrystals for food-related applications using in vivo and in vitro strategies, Ede, J.D., Ong, K.J., Mulenos, M.R., Pradhan, S., Gibb, M., Sayes, C.M., Shatkin, J.A. (2020). Toxicology Research, 9(6): 808-822.

Nano and Sustainable Materials

• Limitations of recent cellulose studies: How overzealous dispersion technique, fluorescence labeling and interpretation limit the findings of recent safety assessments for cellulose materials, Zhang, Y., Fox, D.M., Ong, K.J., Ede, J.D., Sayes, C.M., Kangas, H., Ilona, L., Goergen, M., Moon R., Shatkin, J.A., (2024) Trends in Food Science & Technology, 151, 104620

• The impact of surface functionalization of cellulose following simulated digestion and gastrointestinal cell-based model exposure, Amanda K. Charlton-Sevcik, A.K., Collom, C., Liu J.Y., Hsieh Y-L, Stark, N., Ede, J.D., Shatkin, J.A., Sayes, C.M., (2024) International Journal of Biological Macromolecules, 271(2), 132603

• Latest Developments in the Risk Assessment of Nanomaterials: A Framework for Advanced Materials and Emerging Technologies, Shatkin, J.A., Ede, J.D., Ong, K.J. (2024) Chapter 15 in Human and Ecological Risk Assessment: Theory and Practice, edited by Dennis J. Paustenbach, Kenneth Feinberg, published by Wiley.

• Aqueous exfoliation and dispersion of monolayer and bilayer graphene from graphite using sulfated cellulose nanofibrils, Pingrey, B., Ede, J.D., Sayes, C.M., Shatkin, J.A., Stark, N., Hsieh, Y.-L. (2024) RSC Advances, 2024, 14, 9860-9868.

• Regioselectively Carboxylated Cellulose Nanofibril Models from Dissolving Pulp: C6 via TEMPO Oxidation and C2,C3 via Periodate–Chlorite Oxidation, Guo, M., Ede, J.D., Sayes, C.M., Shatkin, J.A., Stark, N., Hsieh, Y.-L. (2024) . Nanomaterials 2024, 14, 479.

• A comparative analysis of microfibrillated versus conventional cellulose after enzymatic digestions, Collom, C., Fox, D., Ong, K.J. Shatkin, J.A., Sayes, C.M. (2024) Cellulose, Volume 31, pages 5981–5985.

• Life-cycle risk assessment of graphene-enabled textiles in fire protection gear, Ede, J.D., Zhang, Y., Diges, A.S., Shatkin, J.A. (2024) NanoImpact, 43: 100488.

• Review on Hybrid Reinforced Polymer Matrix Composites with Nanocellulose, Nanomaterials, and Other Fibers, Seydibeyoğlu, M.Ö., Dogru, A., Wang, J., Rencheck, M.; Han, Y., Wang, L., Seydibeyoğlu, E.A., Zhao, X., Ong, K.J., Shatkin, J.A., et al. (2023). Polymers 2023, 15, 984.

• Characterization of a Human In Vitro Intestinal Model for the Hazard Assessment of Nanomaterials Used in Cancer Immunotherapy, Gibb, M., Pradhan, S.H., Mulenos, M.R., Lujan, H., Liu, J., Ede, J.D., Shatkin, J.A., Sayes, C.M. (2021). . Appl. Sci. 11: 2113.

• Fluorescently Labeled Cellulose Nanofibers for Environmental Health and Safety Studies, Patel, I., Woodcock, J., Beams, R., Stranick, S.J., Nieuwendaal, R., Gilman, J.W., Mulenos, M.R., Sayes, C.M., Salari, M., DeLoid, G., Demokritou, P., Harper, B., Harper, S., Ong, K.J., Shatkin, J.A., Fox, D.M. (2021).. Nanomaterials, 11: 1015.

• Physical, chemical, and toxicological characterization of sulfated cellulose nanocrystals for food-related applications using in vivo and in vitro strategies, Ede, J.D., Ong, K.J., Mulenos, M.R., Pradhan, S., Gibb, M., Sayes, C.M., Shatkin, J.A. (2020). . Toxicology Research, 9(6): 808-822.

• Translating Scientific Advances in the AOP Framework to Decision Making for Nanomaterials, Ede, J.D., Lobaskin, V., Vogel, U., Lynch, I., Halappanavar, S., Doak, S.H., Roberts, M.G., Shatkin, J.A. (2020).  Nanomaterials, 10(6): 1229.

• A 90-day dietary study with fibrillated cellulose in Sprague-Dawley rats, Ong, K.J., Ede, J.D., Pomeroy-Carter, C.A., Sayes, C.M., Mulenos, M.R., Shatkin, J.A., (2020). . Toxicology Reports, 7: 174-182.

• The Future in Nanosafety, Shatkin, J.A. (2020). Nano Letters, 20(3): 1479-1480.

• Physical, chemical and toxicological characterization of fibrillated forms of cellulose using an in vitro gastrointestinal digestion and co-culture model, Pradhan S.H., Mulenos M.R., Steele L.R., Gibb M., Ede J.D., Ong K.J., Shatkin J.A., and Sayes C.M. (2020). Toxicology Research, 9(3): 290-301.

• A methodology for developing key events to advance nanomaterial-relevant adverse outcome pathways to inform risk assessment, Halappanavar, S., Ede, J.D., Mahapatra, I., Krug, H.F., Kuempel, E.D., Lynch, I., Vandebriel, R.J., Shatkin, J.A. (2020). Nanotoxicology.

• Risk Analysis of Cellulose Nanomaterials by Inhalation: Current State of Science, Ede, J.D., Ong, K.J., Goergen, M., Rudie, A., Pomeroy-Carter, C.A., Shatkin, J.A. (2019). Nanomaterials, 9(3): 337.

• A systematic process for identifying key events for advancing the development of nanomaterial relevant adverse outcome pathways, Halappanavar, S., Ede, J. D., Shatkin, J.A., Krug, H.F. (2019). NanoImpact, 21: 100286.

• Evaluating mucoadhesion properties of three types of nanocellulose in the gastrointestinal tract in vitro and ex vivo, Lin, Y.J., Shatkin, J.A., and Kong. F. (2019). Carbohydrate Polymers, 210: 157-166.

• Collection of airborne ultrafine cellulose nanocrystals by impinger with an efficiency mimicking deposition in the human respiratory system, Roberts, R., Gettz, K., Stebounova, L.V., Peters, T., Shatkin, J.A., Foster, J.E. (2018). . Journal of Occupational and Environmental Hygiene, 16(2): 141-150.

• Review: Current characterization methods for cellulose nanomaterials, Foster E.J., Moon R.J., Agarwal U.P., Bortner M.J., Bras J., Camarero-Espinosa S., Chan K.J., Clift M.J.D., Cranston E.D., Eichhorn S.J., Fox D.M., Hamad W.Y., Heux L., Jean B., Korey M., Nieh W., Ong K.J., Reid M.S., Renneckar S., Roberts R., Shatkin J.A., Simonsen J., Stinson-Bagby K., Wanasekara N., Youngblood J. (2018). Chemical Society Reviews, 47(8): 2609-2679.

• Minimizing Risk: an overview of risk assessment and risk management of nanomaterials, Shatkin, J.A., Ong, K.J., Ede, J.D. (2017). Metrology and Standardization of Nanomaterials: Protocols and Industrial Innovations. Metrology and Standardization of Nanotechnology: Protocols and Industrial Innovations, 24.

• Environmental Health and Safety of Cellulose Nanomaterials and Composites, Shatkin, J.A., Kim, B. (2017). Handbook of Cellulose Nanocomposites, 21.

• Advancing risk analysis for nanoscale materials: Report from an international workshop on the role of alternative testing strategies for advancement, Shatkin, J.A., Ong, K.J., Beaudrie, C., Clippinger, A.J., Hendren, C.O., Haber, L.T., Hill, M., Holden, P., Kennedy, A.J., Kim, B., MacDonell, M. (2016). Risk Analysis, 36(8), 1520-1537.

• Alternative Testing Strategies for Nanomaterials: State of the Science and Considerations for Risk Analysis, Shatkin, J.A. and Ong, K.J. (2016). Risk Analysis: An International Journal, 36(8), 1564-1580.

• The role of the food matrix and gastrointestinal tract in the assessment of biological properties of ingested engineered nanomaterials (iENMs): State of the science and knowledge gaps, McClements, D.J., DeLoid, G., Pyrgiotakis, G., Shatkin, J.A., Xiao, H., Demokritou, P. (2016). NanoImpact, (3-4); 47-57.

• Toward Cellulose Nanomaterial Commercialization: Knowledge Gap Analysis for Safety Data Sheets According to the Globally Harmonized System. Shatkin, J.A., Ong, K.J., Ede, J.D., Wegner, T.H., Goergen, M. (2016). TAPPI Journal, 15(6): 425-437.

• Life Cycle Risk Assessment, and Environmental Health and Safety Roadmap, Shatkin, J.A. and Kim, B. (2015). Cellulose Nanomaterials: Environmental Science: Nano, 2: 477-499. https://doi.org/10.1039/C5EN00059A

• Production of Low Cost Nanocellulose for Renewable, Advanced Materials Applications, Nelson K., Retsina T., Iakovlev M., van Heiningen, A., Deng Y., Shatkin, J.A., and Mulyadi A. (2016).  Materials Research for Manufacturing: An Industrial Perspective of Turning Materials into New Products, 224: 267-302. https://doi.org/10.1007/978-3-319-23419-9_9

• Market projections of cellulose nanomaterial-enabled products -Part 1: Applications, Shatkin, J.A., Wegner, T.H., Bilek, E.M., Cowie, J. (2014). Tappi Journal, 13: 9-16. https://doi.org/10.32964/TJ13.5.9

• Market Projections of cellulose nanomaterial-enabled products Part 2. Volume Estimates, Cowie, J., Bilek, E.M., Wegner, T.H., Shatkin, J.A., (2014). TAPPI Journal, 13 (6):57-69. https://doi.org/10.32964/TJ13.6.57

• A Multi-Stakeholder Perspective on the Use of Alternative Test Strategies for Nanomaterial Safety Assessment, Nel, A.E., Nasser, E., Godwin, H., Avery, D., Bahadori, T., Bergeson, L., Beryt, E., Bonner, J.C., Boverhof, D., Carter, J., Castranova, V., Deshazo, J.R., Hussain, S.M., Kane, A.B., Klaessig, F., Kuempel, E., Lafranconi, M., Landsiedel, R., Malloy, T., Miller, M.B., Morris, J., Moss, K., Oberdorster, G., Pinkerton, K., Pleus, R.C., Shatkin, J.A., Thomas, R., Tolaymat, T., Wang, A., Wong, J. (2013). ACS Nano, 7(8):6422-33. https://doi.org/10.1021/nn4037927.

• Potential release scenarios for carbon nanotubes used in composites, Nowack, B., David, R.M., Heinz Fissan, H., Morris, H., Shatkin, J.A., Stintz, M., Zepp, R., Brouwer, D. (2013). Environment International, 59: 1–11. https://doi.org/10.1016/j.envint.2013.04.003

• Incorporating Life Cycle Thinking into Risk Assessment for Nanoscale Materials: Case Study of Nanocellulose, Shatkin, J.A., Wegner, T., and Nieh, W. (2013). Chapter 1.2 Health Safety and Environment, in, Postek, MT, RJ Moon, AW Rudie and MA Bilodeau, “Production and Applications of Cellulose Nanomaterials”. TAPPI PRESS. ISBN: 978-1-59510-224-9.

• Health, Safety and Environmental Issues, Shatkin, J.A. and Gross, G. (2012). Chapter 10 in: “Nanoscience and Nanomaterials Synthesis, Manufacturing and Industry Impacts” K. Zhong, B. Li, R. G. Maguire, V. Dang, J. Shatkin, G. Gross, and M.C. Richey, Eds. DEStech Publications, Lancaster PA. 

• Shatkin, J.A. (2012). Nanotechnology Health and Environmental Risks Second Edition. Taylor and Francis/CRC Press. Boca Raton, Florida. ISBN-13: 978-1439881750.

• Nanotechnology: What Are the Challenges and Emerging Benefits for Water Treatment?,Tuccillo, M.E., Boyd, G.R., Sandvig, A., Shatkin, J.A., and Dionysiou, D.D. (2012). OpFlow, 38(1). 10-13. https:/./doi.org/10.5991/OPF.2012.38.0001.

• Are Nanotechnology-based Products Safe? Shatkin, J.A. (2012). Nutrition Today, 47(1): 30-32. https://doi.org/10.1097/NT.0b013e3182435c79

• Perspectives on Risks of Nanomaterials and Nanotechnologies: Advancing the Science. Introduction to the Special Series. Shatkin, J.A. and North, W. (2010). Risk Analysis, 30(11): 1627. https://doi.org/10.1111/j.1539-6924.2010.01541.x

• Nano Risk Analysis: Advancing the Science for Nanomaterials Risk Management, Shatkin, J.A., Abbott, L.C., Bradley, A.E., Canady, R.A., Guidotti, T., Kulinowski, K.M., Lofstedt, R.E., Louis, G., MacDonell, M., Maynard, A.D., White, R.D., Williams, R. (2010). Risk Analysis, 30(11): 1680. https://doi.org/10.1111/j.1539-6924.2010.01493.x

• Nanomaterial case studies: nanoscale titanium dioxide in water treatment and in topical sunscreen (Final), Davis, J.M., Wang, A., Shatkin, J.A., Graham, J.A., Gwinn, M. and Ranalli, B., (2010). U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-09/057F.

• Humic acid ameliorates nanoparticle-induced developmental toxicity in zebrafish, Ong, K.J., Felix, L.C., Boyle, D., Ede, J.D., Ma, G., Veinot, J., Goss, G. (2016). Environmental Science: Nano, https://doi.org./10.1039/C6EN00408C.

• Aquatic toxicity of manufactured nanomaterials: Challenges and recommendations for future toxicity testing, Schultz, A.G., Boyle, D., Chamot, D., Ong, K.J., Wilkinson, K., McGeer, J.C., Sunahara, G., Goss, G.G. (2014). Review. Environmental Chemistry, 11(3), 207-226. https://doi.org/10.1071/EN13221

• Widespread nanoparticle-assay interference: Implications for nanotoxicity testing, Ong K.J., MacCormack, T.J., Clark, R.J., Ede, J.D., Ortega, V.A., Felix, L.C., Dang, M.K.M., Ma, G., Fenniri, H., Veinot, J.G.C., Goss, G.G. (2014).  PLoS ONE, 9(3), e90650. https://doi.org/10.1371/journal.pone.0090650

• Mechanistic insights into the effect of nanoparticles on zebrafish hatch, Ong, K.J., Zhao, X., Thistle, M.E., MacCormack, T.J., Clark, R.J., Ma, G., Martinez-Rubi, Y., Simard, B. Loo, J.S.C., Veinot, J.G.C., Goss, G.G. (2013). Nanotoxicology. https://doi.org/10.3109/17435390.2013.778345

• Evaluating the toxicity of hydroxyapatite nanoparticles in catfish cells and zebrafish embryos, Zhao, X., Ong, K.J., Ede, J.D., Stafford, J., Ng, K.W., Goss, G.G., Loo, S.C.J. (2013) Small, 27(9-10). 1734-1741. https://doi.org/10.1002/smll.201200639

• Silver nanoparticles inhibit sodium uptake in juvenile rainbow trout (Oncorhyncus mykiss), Schultz, A.G., Ong, K.J., MacCormack, T.J., Ma, G., Veinot, J.G.C., Goss, G.G. (2012) Environmental Science and Technology, 46(18), 10295-10301. https://doi.org/10.1021/es3017717

• Metabolism, nitrogen excretion, and heat shock proteins in the central mudminnow (Umbra limi), a facultative air-breathing fish living in a variable environment, Currie, S., Bagatto, B., DeMille, M., Learner, A., LeBlanc, D., Marks, C., Ong, K.J., Parker, J., Templeman, N., Tufts, B., Wright, P. (2010) Canadian Journal of Zoology, 88:43-58. https://doi.org/10.1139/Z09-117

• Gill morphology of the mangrove killifish is plastic and changes in response to terrestrial air exposure, Ong K.J., Stevens, E.D., and Wright, P.A. (2007) Journal of Experimental Biology, 210, 1109-1115. https://doi.org/10.1242/jeb.002238

• Establishing the safety of novel bio-based cellulose nanomaterials for commercialization, Ong, K.J., Shatkin, J.A., Nelson, K., Ede, J.D., Retsina, T. (2017). NanoImpact, 6:19-29. https://doi.org/10.1016/j.impact.2017.03.002

• Carbon nanotubes diminish IgE-mediated degranulation in the rat basophilic leukemia (RBL)-2H3 cell line, Ede, J.D., Ortega, V.O., Boyle, D., Felix, L., Stafford, J.L., Martinez-Rubi, Y., Simard, B., Goss, G.G. Carbon, 9:31-41. https://doi.org/10.1016/j.impact.2017.10.001

• Physicochemical properties of functionalized carbon-based nanomaterials and their toxicity to fishes, Felix, L., Ede, J.D., Snell, D.A., Oliveira, T.M., Martinez-Rubi, Y., Simard, B., Luong, J.H.T., Goss, G.G. (2016). Carbon, 104: 78-89. https://doi.org/10.1016/j.carbon.2016.03.041

• Functionalization changes the effects of rosette nanotubes on channel catfish (Ictalurus punctatus) lymphocyte viability and function, Ede, J.D., Ortega, V.O., Boyle, D., Beingessner, R., Hemraz, U., Fenniri, H., Stafford, J., Goss, G.G. (2016) Environmental Science Nano, 3: 578-592. https://doi.org/10.1039/C6EN00024J

• Rosette nanotubes alter IgE-mediated degranulation in the rat basophilic leukemia (RBL)-2H3 cell line, Ede, J.D., Ortega, V.O., Boyle, D., Beingessner, R., Hemraz, U., Fenniri, H., Stafford, J., Goss, G.G. (2015). Toxicological Sciences, 148(1):108-20. https://doi.org/10.1093/toxsci/kfv166

• Polymer-coated Metal-Oxide Nanoparticles Inhibit IgE Receptor Binding, Cellular Signaling, and Degranulation in a Mast Cell-like Line, Ortega, V.A., Ede, J.D., Boyle, D., Stafford, J., Goss, G.G. (2015) Advanced Science, 2(11): 1500104. http://doi.org/10.1002/advs.201500104

• Widespread Nanoparticle-Assay Interference: Implications for Nanotoxicity Testing, Ong, K.J., MacCormack, T.J., Clark, R.J., Ede, J.D., Felix, L., Ortega, V., Dang, M.K.M., Ma, G., Veinot, J.G.C., Goss, G.G. (2014)  PloS One, 9(3): e90650. https://doi.org/10.1371/journal.pone.0090650

• Physicochemical characteristics of polymer-coated metal-oxide nanoparticles and their toxicological effects on zebrafish (Danio rerio) development, Felix, L.C., Ortega, V.A., Ede, J.D., Goss, G.G. (2013)  Environmental Science and Technology, 47(12):6589–6596. https://doi.org/10.1021/es401403p

• Evaluating in vitro and in vivo biocompatibility of hydroxyapatite nanoparticles in catfish cells and zebrafish embryo models, Zhao, X., Ong, K.J., Ede, J.D., Stafford, J., Ng, K.W., Goss, G.G., Loo, S.C.J. (2012) Small, 9:(9-10): 1734-1741. https://doi.org/10.1002/smll.201200639

• Physiological responses of postprandial red rock crabs, Cancer products, during emersion, McGaw, I.J., Curtis, D.L., Ede, J.D., Ong, K.J., Breukelen, F.V., Goss, G.G. (2009)  Canadian Journal of Zoology, 87(12): 1158-1169. https://doi.org/10.1139/Z09-106

For a complete list of publications authored by Dr. Jo Anne Shatkin, download her publication list here.

Recent Presentations

Kimberly Ong joined Lisa M. Keefe, Editor in Chief Alt Meat, on the MeatingPod podcast in a discussion focused on the changes in regulatory approval and consumer acceptance of cultivated meat. You can join the alt-meat.net community for free and listed to the podcast here: https://www.alt-meat.net/meatingpod-guide-cultured-meat-safety-regulatory-approval

Solving for Safety, Presentation from the New Harvest 2022 Cellular Agriculture Conference. View on the New Harvest YouTube channel.

Assessing the Safety of Cellulosic Nanomaterials: A Conversation with Jo Anne Shatkin, 2022. Listen on NanoTube - The National Nanotechnology Initiative Podcast.

TAPPI Nano International Conference on Nanotechnology for Renewable Materials: Establishing the Safety of Cellulose Nanomaterials for Food Related Uses. 2017. Download the presentation, see here. 

Aaron Brody Distinguished Lecture in Food Packaging: Safety of Nanomaterials in the Life Cycle of Food Packaging. 2016.  Presented at Michigan State University, to view the full presentation, see here.

"Building a community of competitors to accelerate safe innovation" by Dr. Kimberly Ong